| 1. | First , to enrich the selective markers for u32 , two resistance genes emr and cat , one report gene amy were introduced into u32 to test their expression
首先对适用于u32的选择性标记进行了筛选，结果表明来自puc19e的红霉素抗性基因和来自pulam2的淀粉酶基因可以作为u32的选择性标记。 |
| 2. | Bar gene also is one of widely used selective marker gene . the cloning of bar gene is important to plant transgenic engineering , gene expression and studying physiology
而且bar基因也是迄今为止应用最为广泛的一个抗除草剂选择标记基因，克隆出bar基因对植物的遗传转化，基因表达和生理学研究都有重要的作用。 |
| 3. | We also conducted the co - transfection with the rnai plasmids rhe and rhc and the selective marker gfp expressing plasmids simultaneously . there are three kinds of combination probability that could be observed by gfp marker
在实验中同时共转染人集缩素smc亚基hcap一e和hcap一c特异的rnai质粒rhe和rhc和pcdna3 . 1 + kg到hela细胞。 |
| 4. | To identify the transfected cells , we also constructed the gfp expressing plasmids which also attached the kozac sequence and nuclear localization signal to the gfp gene and the plasmids served as a selective marker for the transfected cells
为了区分被转染的细胞，以附加kozac序列和核定位信号的gfp ( pcdna3 . 1 + kg )作为被转染的细胞的标记。 |
| 5. | The results indicate that emr and amy can be selective markers for u32 . then with promoter - less amy , promoter - probe pzcamyp " was constructed and fusion expression of glna promoter with promoter - less amy was achieved in u32
然后又以淀粉酶基因为报告基因构建了启动子探针pzcamyp ~ - ，测试结果表明该质粒对u32glna基因的启动子十分敏感，可以作为u32的启动子探针。 |
| 6. | In the second part of the thesis , we described that a tobacco chloroplast expression vector , ptrvp1 , containing the foot and mouth disease virus ( fmdv ) vp1 gene and the selective marker aada gene was constructed and transfered to the tobacci chloroplast genome by the biolistic method
论文第二部分主要叙述了烟草叶绿体表达载体ptrvp1的构建，并通过基因枪方法转化烟草叶绿体基因组，获得了3株具有壮观霉素抗性的转化再生植株。 |
| 7. | No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page , except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ) , which is a selective marker of the wild - type virus . elisa test results suggested the expression of egf in cells , but not in culture supernant . the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract
将重组病毒rbmbacph - egf以10moi感染bmn细胞， 72小时后收集培养细胞和上清；培养上清和经超声波处理的细胞样品elisa检测发现胞内样品中存在能与egf抗体免疫反应的产物，粗略估计表达量约6 7 g 2 10 ~ 6个细胞(相当于egf标准品) ；重组病毒rbmbacph - egf穿刺接种5龄家蚕幼虫，每隔24h收集蚕血淋巴，经elisa检测发现第4天表达量最高，根据egf标准曲线计算蚕血淋巴的表达量约32 g ml ； elisa定性实验还发现正常蚕血也存在与egf抗体间交叉反应的物质。 |
| 8. | Three chloroplast transformation vectors including pds16s - cat , ptn1269 - bar and psp72 - n5 - bar - n3 were constructed , using ! 6s rrna or chln gene sequence as a homologous segment and cat or bar as a selective marker gene , respectively . foreign genes were introduced to the cells of d . salina by microprojectile bombardment method and a pilot chloroplast tran
3 .杜氏盐藻叶绿体165出na基因的克隆和转化载体的构建根据杜氏盐藻的近缘藻类的叶绿体基因组序列资料，克隆了杜氏盐藻叶绿体16srrna基因部分序列1100bp ，并利用克隆的16srrna郑州大学2003年博士学位论文 |
| 9. | 2 . an anther specific chimaeric male sterile gene expression box with a enhanced promoter ( ta29 ) driving coda gene was constructed and the expression box was inserted into binary vector p3301 that contains a l - phosphinothricin ( ppt ) - resistant selective marker gene and - glucuronidase ( gus ) reporter gene in t - dna region
以增强的ta29启动子驱动克隆的coda基因，构建成花药特异性嵌合基因表达盒；将此表达盒插入双元载体p3301 ，构建成以ppt抗性基因为选择标记，以gus为报告基因的植物表达载体。 |
